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Educational Tool
Guide RNA Designer
Enter a DNA sequence or select a preset gene target to identify PAM sites, design guide RNAs, and evaluate their quality. Learn the principles behind CRISPR guide design.
CRISPR System
Streptococcus pyogenes Cas9 — the most widely used CRISPR nuclease. Recognizes NGG PAM on the 3’ side of the target.
DNA Sequence
Load a preset gene target:
Educational Use Only
This is an educational tool demonstrating guide RNA design principles. For actual laboratory experiments, use validated tools like CRISPOR, Benchling, or Cas-OFFinder that include genome-wide off-target analysis.